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中国癌症防治杂志

中国癌症防治杂志 ›› 2013, Vol. 5 ›› Issue (2): 92-95.doi: 10.3969/j.issn.1674-5671.2013.02.02

• 基础研究 • 上一篇    下一篇

体内阿霉素干预富集肝癌干细胞的初步研究

  

  1. 广西医科大学研究生学院；广西医科大学病理生理学教研室、病理学教研室；广西医科大学医学科学实验中心；右江民族医学院口腔颌面外科学教研室
  • 出版日期:2013-06-25 发布日期:2013-06-27
  • 通讯作者: 邝晓聪 E-mail:13617715826@139.com
  • 基金资助:

    国家自然科学基金资助项目（30860326）；广西自然科学基金资助项目（0991139）；广西科技创新能力与条件建设项目（桂科攻 0993003C-1）
                          

In vivo enrichment of liver cancer stem cells through adriamycin treatment：a preliminary study

  • Online:2013-06-25 Published:2013-06-27

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摘要： 目的 建立肝癌裸鼠皮下移植瘤动物模型，以癌干细胞的耐药特性为建立富集肝癌干细胞的切入点，在机体环境下进行构建体内肝癌干细胞的富集模式。方法 将BEL-7404人肝癌细胞株的细胞悬液注射裸鼠两侧腋窝皮下，建立皮下移植瘤模型，经阿霉素干预后原代取材，分别以单个细胞克隆形成实验、平板克隆形成实验和悬浮球形成实验，对亲本细胞及经阿霉素干预后的BEL-7404肝癌细胞进行癌干细胞相关细胞学特性的检测。结果 每一代裸鼠皮下移植瘤模型成瘤率为100%，亲本细胞与经体内阿霉素干预后的第一代BEL-7404肝癌细胞的单细胞克隆形成率比较没有明显差异（P＞0.05），而体内干预第二代细胞的单细胞克隆形成率均明显高于亲本细胞和经干预后的第一代细胞（P＜0.05），体内干预第一代细胞平板克隆形成率均明显低于亲本细胞和第二代细胞（P＜0.05）。亲本细胞及经体内阿霉素干预后的BEL-7404肝癌细胞在干细胞培养基中培养均未见有细胞悬浮球形成。结论 经低剂量阿霉素体内干预可作为富集肝癌干细胞的手段之一。

关键词:  , 肝肿瘤, 癌干细胞, 皮下移植瘤模型, 阿霉素

Abstract: Objective To establish a nude mouse model of liver cancer and explore whether drug resistance of cancer stem-like cells in vivo can lead to enrichment of stem-like cells. Methods A subcutaneous tumor model of liver carcinoma was established by subcu-taneously injecting human liver BEL-7404 cell suspensions into both axilla of BALB/C-nu mice.Following adriamycin treatment，primary cultures were established， and both parental and adriamycin-treated cells were studied in assays of single-cell colony formation and sphere cell formation to detect drug resistance and analyze other cytological characteristics. Results The tumor formation rate in this BALB/C-nu subcutaneous tumor model was 100%.There were no significant differences in the rate of single-cell colony formation between BEL-7404 parental liver cells and first-generation cells after adriamycin treatment（P>0.05）.However，second-generation cells showed a significantly higher rate of single-cell colony formation and a significantly lower colony formation rate than parental or first-generation cells（P<0.05）. Non-adherent spheres were not observed in cultures of any of the three cell types. Conclusions Low-dose adriamycin intervention may be a way to enrich for liver cancer stem-like cells in vivo.

Key words: Liver neoplasms, Cancer stem cell, Subcutaneous tumor model, Adriamycin